Reproductive Biotechnology

Associate Professor Mark Nottle Email: mark.nottle@adelaide.edu.au Contact information

Reproductive Biotechnology has an international reputation in the general areas of reproductive biology and the development of associated technologies such as embryo freezing. In collaboration with a number of University, Institute and Hospital Research Groups in Australia as well as overseas, current research is focussed on developing organ, tissue and cell replacement therapies. This work is funded by various agencies including The Australian Research Council, The Juvenile Diabetes Research Foundation, The National Health and Medical Research Council and Industry.

Recent Publications

Nagashima H, Giannakis C, Ashaman RJ, Nottle MB (2003) Sex differentiation and germ cell production in chimeric pigs produced by inner cell mass injection into Blastocysts. Biology of Reproduction ( Epub ahead of print.)

Nottle MB, Beebe LFS, Harrison SJ and Faast RA (2003) New reproductive technologies for production and research in the pig. A review. In “ Manipulating Pig Production IX” pp43-49 Ed JE Patterson. Australasian Pig Science Association (Inc.), Werribee.

Cowan PJ, Shinkel TA, Fisicaro N, GodwinJW, Bernabeu C, Almendro N, Rius C, Lonie AJ, Nottle MB, Wigley PL, Paizis K, Pearse MJ, d'Apice AJF (2003) Targeting gene expression to endothelium in transgenic animals: a comparison of the human ICAM-2, PECAM-1 and endoglin promoters. Xenotransplantation 10: 223-231.

Grupen CG, McIlfatrick SM, Ashman RJ, Boquest AC, Armstrong DT, Nottle MB. (2003) Relationship between donor animals age, follicular fluid steroid content and oocyte developmental competence in the pig. Reproduction Fertility and Development 15: 81-87.

Grupen CG, Nottle MB and Nagashima H (2002) Calcium release at fertilization: Artificially mimicking the response to sperm. Reproduction and Development 48: 313-333.

Boquest AC, Grupen CG, Harrison SH, McIlfatrick SM, Ashaman RJ, d'Apice AJF, Nottle MB (2002) Production of cloned pigs from cultured fetal fibroblast cells. Biology of Reproduction 66: 1283-1287.

Grupen CG, Mau JC, McIlfatrick SM, Maddocks S, and Nottle MB (2002) Effect of 6-Dimethylaminpurine on electrically activated in vitro matured porcine oocytes. Molecular Reproduction and Development 62: 387-396.

Beebe LFS, Cameron RDA, Blackshaw AW, Higgins A and Nottle MB (2002). Piglets born from centrifuged and vitrified early and peri-hatching blastocysts. (2002) Theriogenology 57: 2155-2165

Harrison SJ, Guidolin A, Faast R, Crocker LA, Giannakis C, d'Apice AJF, Nottle MB, Lyons IJ (2002) Efficient generation of a (1,3) Galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer. Transgenic Research 11: 143-150.

Nottle MB, Haskard KA, Verma PJ, Du ZT, Grupen CG, McIlfatrick SM, Ashman RJ, Harrison SJ, Barlow H, Wigley PL, Lyons IG, Cowan PJ, Crawford RJ, Tolstoshev PL, Pearse MJ, Robins AJ and dÁpice AJF (2001) Effect of DNA concentration on transgenesis rates in mice and pigs. Transgenic Research 10: 523-531.

Nottle MB, d'Apice AJF, Cowan PJ Boquest AC, Harrison SJ and. Grupen CG (2002) Transgenic perspectives in xenotransplantation, (2001). Xenotransplantation 9: 306-308.

d'Apice AJF, Nottle MB and Cowan PJ (2001) Genetic modification for xenotransplantation: transgenics and donors. Transplant proceedings 33: 3053-3054.

Nottle MB, Boquest AC, Harrison SJ, Grupen CG, Tolstoshev PT, Cowan PJ and d Ápice AC (2001) Xenotransplantation. A possible solution to the organ donor shortage. In farm animals in research- can we meet the demands of ethics, welfare, science and industry? Eds R Baker, M Fisher and P Hemsworth. Australian and New Zealand Council for the Care of Animals in Research and Teaching (ANZCCART) Adelaide University, Adelaide.

Nottle MB, Grupen CG, Mau JC, Verma PJ, Du ZT, McIlfatrick SM and Ashman RJ (2000) Artificial Activation of porcine oocytes. Proceedings Second Sendai Symposium on Follicular Growth and Oocyte Maturation Ed E Saito Sendai University, Sendai.

Cameron RDA, Beebe LFS, Blackshaw AW, Higgins A and Nottle MB (2000). Piglets born from vitrified early blastocysts using a simple technique . Australian Veterinary Journal 78: 195-198.

Verma PJ, Du ZT, Crocker L, Faast R, Grupen CG, McIlfatrick SM, Ashman RJ, Lyons IG and Nottle MB (2000) Development of porcine nuclear transfer embryos. Molecular Reproduction Development 57: 262-269.

Grupen CG, Verma PJ, Du ZT, SM McIlfatrick, Ashman RJ and Nottle MB (2000) Activation of in vitro and in vivo derived porcine oocytes using multiple electrical pulses. Reproduction, Fertility and Development 11: 457-462.

Cowan PJ, Aminian A, Barlow H, Brown AA, Chen C-G, Fisicaro N, Francis DMA,

Good man DJ, Han W, Kurek M, Nottle MB, Pearse MJ, Salvaris E, d'Apice AJF (2000) Renal xenografts from triple transgenic pigs are not hyperacute rejected but cause coagulopathy in non-immunosupressed baboons. Transplantation 69 2504-2514.